Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity.

Proceedings of the National Academy of Sciences of the United States of America - 30 Sept 2008

Sarkar Casim A, Dodevski Igor, Kenig Manca, Dudli Stefan, Mohr Anja, Hermans Emmanuel, Plückthun Andreas

Abstract excerpt

We outline a powerful method for the directed evolution of integral membrane proteins in the inner membrane of Escherichia coli. For a mammalian G protein-coupled receptor, we arrived at a sequence with an order-of-magnitude increase in functional expression that still retains the biochemical properties of wild type. This mutant also shows enhanced heterologous expression in eukaryotes (12-fold in Pichia pastoris...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to Directed Molecular Evolution

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Intracellular Membranes

Share to Pichia

Share to Protein Engineering

Share to Receptors, G-Protein-Coupled

Share to Selection, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 18812512
DOI: 10.1073/pnas.0803103105
PMCID: PMC2567449

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity.PMID 18812512Read ↗

Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hostsSemantic proximity Read ↗

Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution.Semantic proximity Read ↗

Combinatorial library approaches for improving soluble protein expression in Escherichia coliSemantic proximity Read ↗

Reprogramming G protein coupled receptor structure and function.Semantic proximity Read ↗

Resistance-based directed evolution of nanobodies for higher affinity in prokaryotes.Semantic proximity Read ↗

G-Protein coupled receptors: models, mutagenesis, and drug design.Semantic proximity Read ↗

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.Semantic proximity Read ↗

Dissecting Protein−Protein Interactions Using Directed EvolutionSemantic proximity Read ↗

Conceptual and methodological advances in cell-free directed evolution.Semantic proximity Read ↗

Protein engineering.Semantic proximity Read ↗

Optimizing the Expression of Human Dopamine Receptors in Escherichia coli.Semantic proximity Read ↗

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.