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Article

Engineering a human P2X2 receptor with altered ligand selectivity in yeast.

The Journal of biological chemistry - 1 May 2024

Gardner Elizabeth C, Tramont Caitlin, Bachanová Petra, Wang Chad, Do Hannah, Boutz Daniel R, Kar Shaunak, Zemelman Boris V, Gollihar Jimmy D, Ellington Andrew D

Abstract excerpt

P2X receptors are a family of ligand gated ion channels found in a range of eukaryotic species including humans but are not naturally present in the yeast Saccharomyces cerevisiae. We demonstrate the first recombinant expression and functional gating of the P2X2 receptor in baker's yeast. We leverage the yeast host for facile genetic screens of mutant P2X2 by performing site saturation mutagenesis at residues of...

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Identifiers

PMID: 38556082
DOI: 10.1016/j.jbc.2024.107248
PMCID: PMC11063903

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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