Article

Structural Insights into Branch Site Proofreading by Human Spliceosome

2022-11-07

Abstract excerpt

Selection of the pre-mRNA branch site (BS) by U2 snRNP is crucial to prespliceosome (A complex) assembly. The RNA helicase PRP5 proofreads BS selection; but the underlying mechanism remains unclear. Here we report the atomic structures of two sequential complexes leading to prespliceosome assembly: human 17S U2 snRNP and a cross-exon pre-A complex. PRP5 is anchored on 17S U2 snRNP mainly through occupation of the...

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Literature Corpus work: a494a375-efa3-5fc3-b6a2-5635515864a3
DOI: 10.1101/2022.11.07.515429

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