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Article

Structural insights into how Prp5 proofreads the pre-mRNA branch site.

Nature - 1 Aug 2021

Zhang Zhenwei, Rigo Norbert, Dybkov Olexandr, Fourmann Jean-Baptiste, Will Cindy L, Kumar Vinay, Urlaub Henning, Stark Holger, Lührmann Reinhard

Abstract excerpt

During the splicing of introns from precursor messenger RNAs (pre-mRNAs), the U2 small nuclear ribonucleoprotein (snRNP) must undergo stable integration into the spliceosomal A complex-a poorly understood, multistep process that is facilitated by the DEAD-box helicase Prp5 (refs. 1-4). During this process, the U2 small nuclear RNA (snRNA) forms an RNA duplex with the pre-mRNA branch site (the U2-BS helix), which...

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Identifiers

PMID: 34349264
DOI: 10.1038/s41586-021-03789-5
PMCID: PMC8357632

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Structural insights into how Prp5 proofreads the pre-mRNA branch site.PMID 34349264Read ↗

A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.Semantic proximity Read ↗

Structural Insights into Branch Site Proofreading by Human SpliceosomeSemantic proximity Read ↗

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Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly.Semantic proximity Read ↗

Structure of the yeast spliceosomal postcatalytic P complex.Semantic proximity Read ↗

Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.Semantic proximity Read ↗

Structural basis for dual roles of Aar2p in U5 snRNP assembly.Semantic proximity Read ↗

Structure-function analysis of the 5' end of yeast U1 snRNA highlights genetic interactions with the Msl5*Mud2 branchpoint-binding complex and other spliceosome assembly factors.Semantic proximity Read ↗

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Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interactionSemantic proximity Read ↗

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