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Article

A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.

Genes & development - 1 Jan 2015

Liang Wen-Wei, Cheng Soo-Chen

Abstract excerpt

The DEAD-box RNA helicase Prp5 is required for the formation of the prespliceosome through an ATP-dependent function to remodel U2 small nuclear ribonucleoprotein particles (snRNPs) and an ATP-independent function of unknown mechanism. Prp5 has also been implicated in proofreading the branch site sequence, but the molecular mechanism has not been well characterized. Using actin precursor mRNA (pre-mRNA) carrying...

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Identifiers

PMID: 25561497
DOI: 10.1101/gad.253708.114
PMCID: PMC4281567

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

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A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.PMID 25561497Read ↗

Structural insights into how Prp5 proofreads the pre-mRNA branch site.Semantic proximity Read ↗

Structural Insights into Branch Site Proofreading by Human SpliceosomeSemantic proximity Read ↗

Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing.Semantic proximity Read ↗

Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly.Semantic proximity Read ↗

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.Semantic proximity Read ↗

Competition between the ATPase Prp5 and branch region-U2 snRNA pairing modulates the fidelity of spliceosome assembly.Semantic proximity Read ↗

Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling.Semantic proximity Read ↗

ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8.Semantic proximity Read ↗

Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly.Semantic proximity Read ↗

Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA.Semantic proximity Read ↗

A U1-U2 snRNP interaction network during intron definition.Semantic proximity Read ↗

ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.Semantic proximity Read ↗

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