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Article

Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.

Structure (London, England : 1993) - 4 Jun 2013

Nguyen Thi Hoang Duong, Li Jade, Galej Wojciech P, Oshikane Hiroyuki, Newman Andrew J, Nagai Kiyoshi

Abstract excerpt

The U5 small nuclear ribonucleoprotein particle (snRNP) helicase Brr2 disrupts the U4/U6 small nuclear RNA (snRNA) duplex and allows U6 snRNA to engage in an intricate RNA network at the active center of the spliceosome. Here, we present the structure of yeast Brr2 in complex with the Jab1/MPN domain of Prp8, which stimulates Brr2 activity. Contrary to previous reports, our crystal structure and mutagenesis data...

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Identifiers

PMID: 23727230
DOI: 10.1016/j.str.2013.04.017
PMCID: PMC3677097

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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