Article
A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.
Nature communications - 5 Jul 2016
Boesler Carsten, Rigo Norbert, Anokhina Maria M, Tauchert Marcel J, Agafonov Dmitry E, Kastner Berthold, Urlaub Henning, Ficner Ralf, Will Cindy L, Lührmann Reinhard
Abstract excerpt
The precise role of the spliceosomal DEAD-box protein Prp28 in higher eukaryotes remains unclear. We show that stable tri-snRNP association during pre-catalytic spliceosomal B complex formation is blocked by a dominant-negative hPrp28 mutant lacking ATPase activity. Complexes formed in the presence of ATPase-deficient hPrp28 represent a novel assembly intermediate, the pre-B complex, that contains U1, U2 and...
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