Article
Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly.
Nucleic acids research - 18 Nov 2019
Beier David H, Carrocci Tucker J, van der Feltz Clarisse, Tretbar U Sandy, Paulson Joshua C, Grabowski Nikolai, Hoskins Aaron A
Abstract excerpt
The DEAD-box family of proteins are ATP-dependent, RNA-binding proteins implicated in many aspects of RNA metabolism. Pre-mRNA splicing in eukaryotes requires three DEAD-box ATPases (Prp5, Prp28 and Sub2), the molecular mechanisms of which are poorly understood. Here, we use single molecule FRET (smFRET) to study the conformational dynamics of yeast Prp5. Prp5 is essential for stable association of the U2 snRNP...
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