Article
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity.
Nature biotechnology - 1 Nov 2020
Lazzarotto Cicera R, Malinin Nikolay L, Li Yichao, Zhang Ruochi, Yang Yang, Lee GaHyun, Cowley Eleanor, He Yanghua, Lan Xin, Jividen Kasey, Katta Varun, Kolmakova Natalia G, Petersen Christopher T, Qi Qian, Strelcov Evgheni, Maragh Samantha, Krenciute Giedre, Ma Jian, Cheng Yong, Tsai Shengdar Q
Abstract excerpt
Current methods can illuminate the genome-wide activity of CRISPR-Cas9 nucleases, but are not easily scalable to the throughput needed to fully understand the principles that govern Cas9 specificity. Here we describe 'circularization for high-throughput analysis of nuclease genome-wide effects by sequencing' (CHANGE-seq), a scalable, automatable tagmentation-based method for measuring the genome-wide activity of...
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