Article

Isolation of secreted proteins from <i>Drosophila</i> ovaries and embryos through <i>in vivo</i> BirA-mediated biotinylation

2019-07-05

Abstract excerpt

The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10 -14 -10 -16 ) has permitted this interaction to be used in a wide variety of experimental contexts. The Biotin Acceptor Peptide (BAP), a 15 amino acid motif that can be biotinylated by the E. coli BirA protein, has been fused to proteins of interest, making them substrates for in vivo biotinylation. Here we report on the con...

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Literature Corpus work: a36fb525-eb4d-564d-a226-a36646eb4344
DOI: 10.1101/694091

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