Article
Efficient induction of proximity-dependent labelling by biotin feeding in BMAL1-BioID knock-in mice.
Journal of biochemistry - 4 Dec 2021
Murata Kazuya, Mimura Asuka, Suzuki Hayate, Mikami Natsuki, Hamada Yuko, Kato Kanako, Iki Natsumi, Ishida Miyuki, Daitoku Yoko, Tanimoto Yoko, Okiyoneda Tsukasa, Fujiyama Tomoyuki, Dinh Tra Thi Huong, Mizuno Seiya, Sugiyama Fumihiro
Abstract excerpt
Proximity-dependent biotin identification (BioID) is a useful method to identify unknown protein-protein interactions. Few reports have described genetically engineered knock-in mouse models for in vivo BioID. Thus, little is known about the proper method for biotin administration and which tissues are applicable. Here, we established a BioID knock-in mouse model of Brain and Muscle ARNT-Like 1 (BMAL1) and the...
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