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Functional tagging of endogenous proteins and rapid selection of cell pools (Rapid generation of endogenously tagged <i>piwi</i> in ovarian somatic sheath cells.)

2020-12-20

Abstract excerpt

The combination of genome-editing and epitope tagging provides a powerful strategy to study proteins with high affinity and specificity while preserving their physiological expression patterns. However, stably modifying endogenous genes in cells that do not allow for clonal selection has been challenging. Here, we present a simple and fast strategy to generate stable, endogenously tagged alleles in a non-transform...

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Identifiers and source

Literature Corpus work
9dbfc784-1921-5648-8fc1-244ff431ba9f
DOI
10.1101/2020.12.18.423517
Open publication

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Functional tagging of endogenous proteins and rapid selection of cell pools (Rapid generation of endogenously tagged <i>piwi</i> in ovarian somatic sheath cells.)DOI 10.1101/2020.12.18.423517
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