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Article

AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.

eLife - 11 May 2020

Kido Kohki, Yamanaka Satoshi, Nakano Shogo, Motani Kou, Shinohara Souta, Nozawa Akira, Kosako Hidetaka, Ito Sohei, Sawasaki Tatsuya

Abstract excerpt

Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. However, there have been some improvements in the enzymes that are used for that purpose. Here, we demonstrate a novel BirA enzyme, AirID (ancestral BirA for proximity-dependent biotin identification), which was...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 32391793
DOI: 10.7554/eLife.54983
PMCID: PMC7302878

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