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Article

Promiscuous protein biotinylation by Escherichia coli biotin protein ligase.

Protein science : a publication of the Protein Society - 1 Nov 2004

Choi-Rhee Eunjoo, Schulman Howard, Cronan John E

Abstract excerpt

Biotin protein ligases (BPLs) are enzymes of extraordinary specificity. BirA, the BPL of Escherichia coli biotinylates only a single cellular protein. We report a mutant BirA that attaches biotin to a large number of cellular proteins in vivo and to bovine serum albumin, chloramphenicol acetyltransferase, immunoglobin heavy and light chains, and RNAse A in vitro. The mutant BirA also self biotinylates in vivo and...

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Identifiers

PMID: 15459338
DOI: 10.1110/ps.04911804
PMCID: PMC2286582

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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