Article
Characterization of differentially expressed genes in purified Drosophila follicle cells: toward a general strategy for cell type-specific developmental analysis.
Proceedings of the National Academy of Sciences of the United States of America - 11 May 1999
Bryant Z, Subrahmanyan L, Tworoger M, LaTray L, Liu C R, Li M J, van den Engh G, Ruohola-Baker H
Abstract excerpt
Axis formation in Drosophila depends on correct patterning of the follicular epithelium and on signaling between the germ line and soma during oogenesis. We describe a method for identifying genes expressed in the follicle cells with potential roles in axis formation. Follicle cells are purified from whole ovaries by enzymatic digestion, filtration, and fluorescence-activated cell sorting (FACS). Two strategies...
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