Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The use of BirA-BAP system to study the effect of US2 and US11 on MHC class I heavy chain in cells.

Immunology letters - 1 Oct 2017

Gauthami S, Kumar Deepak, SivaSai K S R, Hegde Nagendra R

Abstract excerpt

Biotinylation has been extensively used for antibody tagging, affinity-based purification, and in protein/DNA-protein interaction studies. Here we describe the use of biotinylation to study the turn-over of proteins in cells. We use the prokaryotic biotin ligase (BirA) to biotinylate the human leukocyte antigen (HLA)-A2 (A2) heavy chain (HC), which was engineered to contain a biotin acceptor peptide (BAP)....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenoviridae
Share to Carbon-Nitrogen Ligases
Share to Cytomegalovirus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Endopeptidase Clp

Share to Genetic Engineering

Share to Heat-Shock Proteins

Share to HLA-A2 Antigen

Share to Proteolysis

Source

Identifiers

PMID: 28860039
DOI: 10.1016/j.imlet.2017.08.027

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The use of BirA-BAP system to study the effect of US2 and US11 on MHC class I heavy chain in cells.PMID 28860039Read ↗

A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substratesSemantic proximity Read ↗

Promiscuous protein biotinylation by Escherichia coli biotin protein ligase.Semantic proximity Read ↗

Human cytomegalovirus-encoded US2 differentially affects surface expression of MHC class I locus products and targets membrane-bound, but not soluble HLA-G1 for degradation.Semantic proximity Read ↗

The pathway of US11-dependent degradation of MHC class I heavy chains involves a ubiquitin-conjugated intermediate.Semantic proximity Read ↗

AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.Semantic proximity Read ↗

Antigen presentation subverted: Structure of the human cytomegalovirus protein US2 bound to the class I molecule HLA-A2Semantic proximity Read ↗

Human Cytomegalovirus US2 Endoplasmic Reticulum-Lumenal Domain Dictates Association with Major Histocompatibility Complex Class I in a Locus-Specific MannerSemantic proximity Read ↗

[Progress in research and application of the biotin ligase BirA and its mutants in pathogen-host interaction].Semantic proximity Read ↗

Amino acid composition of α1/α2 domains and cytoplasmic tail of MHC class I molecules determine their susceptibility to human cytomegalovirus US11‐mediated down‐regulationSemantic proximity Read ↗

Isolation of secreted proteins from <i>Drosophila</i> ovaries and embryos through <i>in vivo</i> BirA-mediated biotinylationSemantic proximity Read ↗

Subtle sequence variation among MHC class I locus products greatly influences sensitivity to HCMV US2- and US11-mediated degradation.Semantic proximity Read ↗

Internalization of the lymphocytic surface protein CD22 is controlled by a novel membrane proximal cytoplasmic motif.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.