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An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway

13 Feb 2023

Abstract excerpt

Background Humoral immunity depends on the differentiation of B cells into antibody secreting cells (ASCs). Excess or inappropriate ASC differentiation can lead to antibody-mediated autoimmune diseases, while impaired differentiation results in immunodeficiency. Methods We have used CRISPR/Cas9 technology in primary B cells to screen for regulators of terminal differentiation and antibody production. Results We...

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PMID: 36860866
DOI: 10.3389/fimmu.2023.1089243

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