Article
Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells.
Molecular therapy : the journal of the American Society of Gene Therapy - 7 Feb 2018
Hung King L, Meitlis Iana, Hale Malika, Chen Chun-Yu, Singh Swati, Jackson Shaun W, Miao Carol H, Khan Iram F, Rawlings David J, James Richard G
Abstract excerpt
The ability to engineer primary human B cells to differentiate into long-lived plasma cells and secrete a de novo protein may allow the creation of novel plasma cell therapies for protein deficiency diseases and other clinical applications. We initially developed methods for efficient genome editing of primary B cells isolated from peripheral blood. By delivering CRISPR/CRISPR-associated protein 9 (Cas9)...
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