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Article

Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins.

Journal of immunological methods - 1 Jun 2018

Wu Chung-An M, Roth Theodore L, Baglaenko Yuriy, Ferri Dario M, Brauer Patrick, Zuniga-Pflucker Juan Carlos, Rosbe Kristina W, Wither Joan E, Marson Alexander, Allen Christopher D C

Abstract excerpt

Genome editing in human cells with targeted nucleases now enables diverse experimental and therapeutic genome engineering applications, but extension to primary human B cells remains limited. Here we report a method for targeted genetic engineering in primary human B cells, utilizing electroporation of CRISPR-Cas9 ribonucleoproteins (RNPs) to introduce gene knockout mutations at protein-coding loci with high...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29614266
DOI: 10.1016/j.jim.2018.03.009
PMCID: PMC6124898

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