Article

Using Ribonucleoprotein-based CRISPR/Cas9 to edit single nucleotide on human induced pluripotent stem cells to model type 3 long QT syndrome (SCN5A+/-)

2023-05-18

Abstract excerpt

<title>Abstract</title> <p>Human induced pluripotent stem cells (hiPSCs) have been widely used in cardiac disease modelling, drug discovery, and regenerative medicine as they can be differentiated into patient-specific cardiomyocytes. Long QT syndrome type 3 (LQT3) is one of the more malignant congenital long QT syndrome (LQTS) variants with an SCN5A gain-of-function effect on the gated sodium channel. Moreover,...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a270363d-4d5a-5293-9eb3-7346e163637e
DOI: 10.21203/rs.3.rs-2928044/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Using Ribonucleoprotein-based CRISPR/Cas9 to edit single nucleotide on human induced pluripotent stem cells to model type 3 long QT syndrome (SCN5A+/-)DOI 10.21203/rs.3.rs-2928044/v1Read

Defining Cardiomyocyte Repolarization Response to Pharmacotherapy in Long-QT Syndrome Type 3.Semantic proximity Read

Generation of human induced pluripotent stem cell line carrying SCN5AC2204>T Brugada mutation (MUSli009-A-1) introduced by CRISPR/Cas9-mediated genome editing.Semantic proximity Read

Base Editing of Human Pluripotent Stem Cells for modeling Long QT syndromeSemantic proximity Read

Generation of a heterozygous SCN5A knockout human induced pluripotent stem cell line by CRISPR/Cas9 edition.Semantic proximity Read

Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes.Semantic proximity Read

CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells.Semantic proximity Read

CRISPR/Cas9-based repair of a heterozygous HNF1A mutation in patient-derived hiPSCs.Semantic proximity Read

Modeling type 3 long QT syndrome with cardiomyocytes derived from patient-specific induced pluripotent stem cells.Semantic proximity Read

P53 toxicity is a hurdle to CRISPR/CAS9 screening and engineering in human pluripotent stem cellsSemantic proximity Read

The Advantages, Challenges, and Future of Human-Induced Pluripotent Stem Cell Lines in Type 2 Long QT Syndrome.Semantic proximity Read

Genome-Scale CRISPR Screening Identifies Novel Human Pluripotent Gene NetworksSemantic proximity Read

Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells.Semantic proximity Read