Article
Generation of human induced pluripotent stem cell line carrying SCN5AC2204>T Brugada mutation (MUSli009-A-1) introduced by CRISPR/Cas9-mediated genome editing.
Stem cell research - 1 Dec 2019
Angsutararux Paweorn, Luanpitpong Sudjit, Chingsuwanrote Pimjai, Supraditaporn Kantpitchar, Waeteekul Supaporn, Terbto Papussorn, Lorthongpanich Chanchao, Laowtammathron Chuti, U-Pratya Yaowalak, Issaragrisil Surapol
Abstract excerpt
Human induced pluripotent stem cells (hiPSCs) derived from dermal fibroblasts having wild type (WT) SCN5A were engineered by CRISPR/Cas9-mediated genome editing to harbor a specific point mutation (C2204>T) in SCN5A, which results in a substitution of the WT alanine by valine at codon 735 (A735V). The established MUSli009-A-1 hiPSC line has a homozygous C2204>T mutation on exon 14 of SCN5A that was confirmed by...
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