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Article

A simple and efficient method for making site-directed mutants, deletions, and fusions of large DNA such as P1 and BAC clones.

Genome research - 1 Nov 1996

Borén J, Lee I, Callow M J, Rubin E M, Innerarity T L

Abstract excerpt

This study addresses two important technical problems: how to perform targeted alterations such as site-directed mutagenesis and deletions in large fragments of DNA and how to construct full-length genes from two partly overlapping bacterial artificial chromosome (BAC) plasmids. Given the size and the lack of convenient unique restriction sites in these large-insert bacterial clones, these are nontrivial tasks....

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Share to Electrophoresis, Gel, Pulsed-Field

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 8938437
DOI: 10.1101/gr.6.11.1123

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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