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Article

REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering.

Scientific reports - 11 Jan 2016

Trehan Ashutosh, Kiełbus Michał, Czapinski Jakub, Stepulak Andrzej, Huhtaniemi Ilpo, Rivero-Müller Adolfo

Abstract excerpt

Mutagenesis is an important tool to study gene regulation, model disease-causing mutations and for functional characterisation of proteins. Most of the current methods for mutagenesis involve multiple step procedures. One of the most accurate methods for genetically altering DNA is recombineering, which uses bacteria expressing viral recombination proteins. Recently, the use of in vitro seamless assembly systems...

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Share to Chromosomes, Artificial, Bacterial
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Mutagenesis, Site-Directed

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Share to Recombination, Genetic

Source

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Identifiers

PMID: 26750263
DOI: 10.1038/srep19121
PMCID: PMC4707547

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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