Article

Dissecting multi-gene virulence phenotypes with base editing requires consideration of target-independent mutagenesis

2026-02-12

Abstract excerpt

Bacterial pathogenicity arises from complex genetic interactions that are difficult to characterise through single-gene deletions. CRISPR base editors can generate multiplexed gene knockouts, yet this technology remains unexplored for dissecting bacterial pathogenicity. Here, we developed a base-editing pipeline for multi-gene knockouts while revealing that target-independent editing can contribute to variability...

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Literature Corpus work: a0207aa1-f289-5561-9787-6f3de48dac76
DOI: 10.64898/2026.02.11.705302

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