Article

Architecture Matters: Design Rules for Multigene IDO1/PD-L1 Cassettes in Human Skin Cells

2026-03-31

Abstract excerpt

Allogeneic cell therapies require the coordinated expression of multiple immunomodulatory genes, yet multigene circuits that function in permissive cell lines often fail in differentiated human tissues for unclear reasons. Here, we systematically dissect how transcriptional architecture governs functional immunoregulation in engineered human keratinocyte and fibroblast lines. Using site-specific large-cargo integr...

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Literature Corpus work: 9cd2200c-fe06-5fc9-848a-9aa34743fd23
DOI: 10.64898/2026.03.28.714644

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