Article

Modular Pooled Discovery of Synthetic Knockin Sequences to Program Durable Cell Therapies

2022-07-27

Abstract excerpt

<h4>SUMMARY</h4> Chronic stimulation can cause T cell dysfunction and limit efficacy of cellular immunotherapies. CRISPR screens have nominated gene targets for engineered T cells, but improved methods are required to compare large numbers of synthetic knockin sequences to reprogram cell functions. Here, we developed Modular Pooled Knockin Screening (ModPoKI), an adaptable platform for modular construction of DNA...

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Literature Corpus work: 76424ee3-4f72-55a2-b426-d3f297927105
DOI: 10.1101/2022.07.27.501186

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