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Article

A general onepot-method for nucleic acid detection with CRISPR-Cas12a

2020-07-24

Abstract excerpt

<title>Abstract</title> <p>CRISPR/Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid, portable and accurate features. In combination with isothermal amplification technology, single-copy sensitivity can be achieved. However, cleavage of the amplicons and primers by the cis- and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection steps into a...

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Literature Corpus work
62ea70b6-944d-5223-aa73-2b45e41b8efc
DOI
10.21203/rs.3.rs-44613/v1
Open publication

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