Article

Saturation mutagenesis map of generalist versus specialist adaptations of β-lactamase to novel antibiotics

2025-10-15

Abstract excerpt

The evolution of β-lactamase proteins is shaped by the need to maintain enzymatic activity against previously prevalent β-lactam antibiotics while expanding substrate range against new classes of antibiotics. Using saturation mutagenesis and sequence-barcoding-based quantification, we comprehensively mapped the response of the fitness landscape of TEM-1 β-lactamase, which evolved against penicillin-class antibioti...

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Identifiers and source

Literature Corpus work: 96aff92c-514e-53bf-aa47-b873ed33f229
DOI: 10.1101/2025.10.14.682469

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