Article
Comprehensive exploration of the translocation, stability and substrate recognition requirements in VIM-2 lactamase.
eLife - 8 Jun 2020
Chen John Z, Fowler Douglas M, Tokuriki Nobuhiko
Abstract excerpt
Metallo-β-lactamases (MBLs) degrade a broad spectrum of β-lactam antibiotics, and are a major disseminating source for multidrug resistant bacteria. Despite many biochemical studies in diverse MBLs, molecular understanding of the roles of residues in the enzyme's stability and function, and especially substrate specificity, is lacking. Here, we employ deep mutational scanning (DMS) to generate comprehensive...
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