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Article

Deep mutational scanning of essential bacterial proteins can guide antibiotic development.

Nature communications - 16 Jan 2023

Dewachter Liselot, Brooks Aaron N, Noon Katherine, Cialek Charlotte, Clark-ElSayed Alia, Schalck Thomas, Krishnamurthy Nandini, Versées Wim, Vranken Wim, Michiels Jan

Abstract excerpt

Deep mutational scanning is a powerful approach to investigate a wide variety of research questions including protein function and stability. Here, we perform deep mutational scanning on three essential E. coli proteins (FabZ, LpxC and MurA) involved in cell envelope synthesis using high-throughput CRISPR genome editing, and study the effect of the mutations in their original genomic context. We use more than...

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Identifiers

PMID: 36646716
DOI: 10.1038/s41467-023-35940-3
PMCID: PMC9842644

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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