Insights into an evolutionary strategy leading to antibiotic resistance.

Abstract excerpt

Metallo-β-lactamases (MBLs) with activity towards a broad-spectrum of β-lactam antibiotics have become a major threat to public health, not least due to their ability to rapidly adapt their substrate preference. In this study, the capability of the MBL AIM-1 to evade antibiotic pressure by introducing specific mutations was probed by two alternative methods, i.e. site-saturation mutagenesis (SSM) of active site...

Topics

Join the communities discussing this publication.

• beta-Lactams
• Biocatalysis
• Catalytic Domain
• Crystallography, X-Ray
• Directed Molecular Evolution