Article

Optimised whole-genome CRISPR interference screens identify ARID1A-dependent growth regulators in human induced pluripotent stem cells

2022-10-03

Abstract excerpt

Perturbation of gene function is a powerful way to understand the role of individual genes in cellular systems. Whole-genome CRISPR/Cas-based screens have parallelized this approach and identified genes that modulate growth in many contexts. However, the DNA break-induced stress upon Cas9 action limits the efficacy of these screens in important models, such as human induced pluripotent stem cells (iPSCs). Silencin...

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Literature Corpus work: 955341ee-0b93-51d4-b76e-b2c1aae36bb0
DOI: 10.1101/2022.10.03.510590

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