Article

Comprehensive and accelerated mapping of driver mutations through single-nucleotide random mutagenesis of target genes

2025-11-05

Abstract excerpt

Identification of novel driver mutations is crucial for personalized medicine and drug discovery. However, genome-wide studies based solely on patient-derived data cannot confirm the oncogenic potential of detected mutations. Here, we present a genome editing-based method for comprehensive random mutagenesis of target genes. This approach generates cultured cells harboring random single-nucleotide variants (SNVs)...

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Literature Corpus work: 9530e7a1-3417-578b-b832-430b346f8aa5
DOI: 10.1101/2025.11.05.686667

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