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Article

Targeted genetic screening in bacteria with a Cas12k-guided transposase.

Cell reports - 31 Aug 2021

Chen Weizhong, Ren Ze-Hui, Tang Na, Chai Guoshi, Zhang Hongyuan, Zhang Yifei, Ma Jiacheng, Wu Zhaowei, Shen Xia, Huang Xingxu, Luo Guan-Zheng, Ji Quanjiang

Abstract excerpt

Microbes employ sophisticated cellular networks encoded by complex genomes to rapidly adapt to changing environments. High-throughput genome engineering methods are valuable tools for functionally profiling genotype-phenotype relationships and understanding the complexity of cellular networks. However, current methods either rely on special homologous recombination systems and are thus applicable in only limited...

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PMID: 34469724
DOI: 10.1016/j.celrep.2021.109635

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