Article

Knock-In of a 25-Kilobase Pair BAC-Derived Donor Molecule by Traditional and CRISPR/ <i>Cas9</i> -Stimulated Homologous Recombination

2016-09-23

Abstract excerpt

<h4>ABSTRACT</h4> Here, we describe an expansion of the DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus. Driving our efforts was the desire to create a whole animal model that would replace 17 kbp of the mouse Bcl2l11 gene with the corresponding 25-kbp se...

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Literature Corpus work: 1036e9a7-30d3-5bb2-ac7c-3f764246063d
DOI: 10.1101/076612

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