Back to search

Article

CRISPR screens in physiologic medium reveal conditionally essential genes in human cells

2020-08-31

Abstract excerpt

<h4>SUMMARY</h4> Forward genetic screens across hundreds of diverse cancer cell lines have started to define the genetic dependencies of proliferating human cells and how these vary by genotype and lineage. Most screens, however, have been carried out in culture media that poorly resemble metabolite availability in human blood. To explore how medium composition influences gene essentiality, we performed CRISPR-ba...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
8d0ae038-40b6-5d30-99e6-192cdc2b8832
DOI
10.1101/2020.08.31.275107
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
CRISPR screens in physiologic medium reveal conditionally essential genes in human cellsDOI 10.1101/2020.08.31.275107
Screening in serum-derived medium reveals differential response to compounds targeting metabolismSemantic proximity
Conditional lethality profiling reveals anticancer mechanisms of action and drug-nutrient interactionsSemantic proximity
The phenotypic landscape of essential human genesSemantic proximity
Functional Human Genes Typically Exhibit Epigenetic ConservationSemantic proximity
Systematic discovery and classification of human cell line essential genesSemantic proximity
The Influence of Micronutrients in Cell Culture: A Reflection on Viability and Genomic StabilitySemantic proximity
High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.Semantic proximity
OGEE v3: Online GEne Essentiality database with increased coverage of organisms and human cell lines.Semantic proximity
Hierarchical organization of the human cell from a cancer coessentiality networkSemantic proximity
High-resolution yeast quiescence profiling in human-like media reveals interacting influences of auxotrophy and nutrient availabilitySemantic proximity
Human Plasma-Like Medium Promotes Maturation of Human Pluripotent Stem Cell-Derived CardiomyocytesSemantic proximity
Evaluating transportability of <i>in-vitro</i> cellular models to <i>in-vivo</i> human phenotypes using gene perturbation dataSemantic proximity
Select a neighboring publication to make it the new centre.