Article

Membrane Proteins at Scale: Automated Copolymer Nanodisc Purification for Structure and Function

2025-09-06

Abstract excerpt

Membrane proteins (MPs) remain among the most important yet least accessible classes of drug targets. Traditional detergent solubilization strips away native lipids, destabilizing proteins and limiting subsequent work. Amphiphilic copolymers offer a powerful alternative, directly extracting MPs in their native lipid environment. However, because each target protein responds differently to individual copolymers, so...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8b0f1d71-bd0d-5b3b-8d36-994937b76dbf
DOI: 10.1101/2025.09.05.674548

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Membrane Proteins at Scale: Automated Copolymer Nanodisc Purification for Structure and FunctionDOI 10.1101/2025.09.05.674548Read

The bigger picture: global analysis of solubilization performance of classical detergents versus new synthetic polymers utilizing shotgun proteomicsSemantic proximity Read

Analytical methodology for unveiling the physical effect of detergents on the function of full-length membrane proteins using a single-molecule tracking systemSemantic proximity Read

Sulfo-DIBMA encapsulation uniquely preserves signalling-competent active states of Class B1 GPCRs, CGRP and PTH1 receptors, in native-like nanodiscsSemantic proximity Read

BzAM terpolymer series with a step-wise systematic change in hydrophobicity, provides a nuanced platform for functional analysis of GPCRs in native-like nanodiscsSemantic proximity Read

A dual sensor regulates P-glycoprotein’s structural plasticitySemantic proximity Read

Fine-Tuning α-Synuclein Phase Separation through Sequence-Optimized Peptide ModulatorsSemantic proximity Read

Ligandability at the membrane interface of GPx4 revealed through a reverse micelle fragment screening platformSemantic proximity Read

The Structure of the Polycystic Kidney Disease Channel PKD2 in Lipid NanodiscsSemantic proximity Read

Peptidisc-assisted hydrophobic clustering towards the production of multimeric and multispecific nanobody proteinsSemantic proximity Read

Understanding, inhibiting, and engineering membrane transporters with high-throughput mutational screens.Semantic proximity Read

Activity and Structural Dynamics of Human ABCA1 in a Lipid Membrane.Semantic proximity Read

Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EMSemantic proximity Read