Article

Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EM

2021-06-25

Abstract excerpt

<h4>ABSTRACT</h4> A major bottleneck in structural biology is producing biologically relevant samples at sufficient quantities. This is particularly true for large protein assemblies where conventional techniques of gene overexpression require substantial optimization, hampering structural studies and drug development efforts. Here we describe a method combining CRISPR/Cas gene editing and fluorescence cell sorti...

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Literature Corpus work: 2fc7373d-eb6a-5dd2-8a31-1d52637cc10c
DOI: 10.1101/2021.06.25.449985

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