Article

Reprogramming Cas9 PAM Recognition for Allele-Specific Editing

2026-08-11

Abstract excerpt

<h4>Summary</h4> The therapeutic potential of CRISPR–Cas9 genome editing is fundamentally constrained by the requirement for specific short DNA sequences (PAMs) flanking the target site, limiting access to many clinically relevant genomic loci. This stringent PAM requirement is particularly problematic in applications which require precise positioning, such as base editing and allele-specific editing. Although PA...

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Literature Corpus work: 7523629f-2087-5497-a7bb-357b3f7889b9
DOI: 10.64898/2026.08.10.744034

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