Article

Optimization of molecular tools for high-throughput genetic screening

2018-01-01

Abstract excerpt

Forward genetic screening allows for the identification of any genes important for a particular biological process or phenotype. While the power of this approach is broadly agreed on, the efficacy of currently available tools limits the strength of conclusions drawn from these experiments. This thesis describes a method to optimize molecular tools for high-throughput screening, both for shRNA and sgRNA based reage...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 88c9bbc8-f960-5d4b-8fe3-3465df401707
DOI: 10.17863/cam.18903

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Optimization of molecular tools for high-throughput genetic screeningDOI 10.17863/cam.18903Read

dbGuide: A database of functionally validated guide RNAs for genome editing in human and mouse cellsSemantic proximity Read

An Isogenic Cell Line Panel for Sequence-based Screening of Targeted Anti-cancer DrugsSemantic proximity Read

GUIDES: sgRNA design for loss-of-function screensSemantic proximity Read

Using pooled miR30-shRNA library for cancer lethal and synthetic lethal screens.Semantic proximity Read

A mammalian functional-genetic approach to characterizing cancer therapeutics.Semantic proximity Read

A CRISPR-drug perturbational map for identifying new compounds to combine with commonly used chemotherapeuticsSemantic proximity Read

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.Semantic proximity Read

A large-scale resource for tissue-specific CRISPR mutagenesis in <i>Drosophila</i>Semantic proximity Read

Systematic empirical evaluation of individual base editing targets: Validating therapeutic targets in USH2A and comparison of methods.Semantic proximity Read

Identifying chemogenetic interactions from CRISPR knockout screens with drugZSemantic proximity Read

Minimized double guide RNA libraries enable scale-limited CRISPR/Cas9 screensSemantic proximity Read

CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infectionSemantic proximity Read