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Article

Using pooled miR30-shRNA library for cancer lethal and synthetic lethal screens.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Lee Liam Changwoo, Gao Shaojian, Li Qiuning, Luo Ji

Abstract excerpt

Pooled shRNA library is a powerful, rapid, and cost-effective technology to carry out functional genomic screens in mammalian cells. This approach has been applied extensively to identify genetic dependencies in cancer cells that might be exploited for therapeutic purposes. In this chapter we provide a detailed protocol for using the Hannon-Elledge miR30-based library to conduct dropout screens in cancer cell...

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PMID: 25030918
DOI: 10.1007/978-1-4939-0992-6_5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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