One-Pot Isothermal Linear Amplification and Cas12a-based Nucleic Acid Detection

Abstract excerpt

CRISPR-based nucleic acid diagnostics are a promising class of point-of-care tools that could dramatically improve healthcare outcomes for millions worldwide. However, these diagnostics require nucleic acid pre-amplification, an additional step that complicates deployment to low resource settings. Here, we developed CATNAP ( Ca s t rans - n uclease detection of a mplified p roducts), a method that integrates...

Topics

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• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Genotype
• Nucleic Acid Amplification Techniques
• Papillomaviridae
• Papillomavirus Infections
• Polymorphism, Single Nucleotide
• Transposases

Identifiers and source

Literature Corpus work

1958c50b-c10c-54c4-b404-da2d1e9cd478

DOI

10.1101/2025.06.27.661965