Article
Enhanced SfaTnpB enables single-base-specific, one-pot nucleic acid detection for high-sensitivity diagnostics.
Nucleic acids research - 5 Jan 2026
Xu Bingrong, Li Sheng, Li Yong, Zhao Shuhong, Li Xinyun, Han Jianlin, Wu Di, Li Shuaicheng, Chen Ling, Xie Shengsong, Han Xiaosong, Zhao Changzhi
Abstract excerpt
CRISPR/Cas12-based nucleic acid detection has revolutionized molecular diagnostics but shows limited single-nucleotide specificity, limited high-fidelity subtype discrimination, and limited compatibility with one-pot assays, restricting its broader clinical application. Here, we report a transposon-associated transposase B (TnpB) ortholog, SfaTnpB, with high trans-cleavage activity, robust single-base mismatch...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.