Article

C-terminal tagging impairs AGO2 function

2023-11-20

Abstract excerpt

A full understanding of RNA silencing requires appropriate molecular biology tools to explore the roles of Argonaute 2 (AGO2) and the RNA-induced silencing complex (RISC). Approaches relying on affinity tagging and antibodies have important limitations that can lead to artificial results. Both the N- and C-terminal domains of AGO2 have been shown to be important for correct activity and yet the consequences of app...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 88807cd2-448a-5188-bd79-86f9b48f9560
DOI: 10.1101/2023.11.20.567203

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

C-terminal tagging impairs AGO2 functionDOI 10.1101/2023.11.20.567203Read

Germline AGO2 mutations impair RNA interference and human neurological development.Semantic proximity Read

High-resolution <i>in vivo</i> identification of miRNA targets by Halo-Enhanced Ago2 PulldownSemantic proximity Read

A dicer-independent miRNA biogenesis pathway that requires Ago catalysis.Semantic proximity Read

Second-Site Mutagenesis of a Hypomorphic argonaute1 Allele Identifies SUPERKILLER3 as an Endogenous Suppressor of Transgene Posttranscriptional Gene Silencing.Semantic proximity Read

Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes.Semantic proximity Read

An AGO2 adaptor expands the functional and evolutionary reach of microRNA targetingSemantic proximity Read

Dysregulation of AGO2-miRNA dynamics underlies the AGO2-associated Lessel-Kreienkamp syndrome.Semantic proximity Read

Programmed Manipulation of RNA Targets By Human Argonaute 2Semantic proximity Read

Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing.Semantic proximity Read

Natural variation of the amino-terminal glutamine-rich domain in Drosophila argonaute2 is not associated with developmental defects.Semantic proximity Read

C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.Semantic proximity Read

A genetic screen for components of the mammalian RNA interference pathway in Bloom-deficient mouse embryonic stem cells.Semantic proximity Read