Article
Second-Site Mutagenesis of a Hypomorphic argonaute1 Allele Identifies SUPERKILLER3 as an Endogenous Suppressor of Transgene Posttranscriptional Gene Silencing.
Plant physiology - 1 Oct 2015
Yu Agnès, Saudemont Baptiste, Bouteiller Nathalie, Elvira-Matelot Emilie, Lepère Gersende, Parent Jean-Sébastien, Morel Jean-Benoit, Cao Jun, Elmayan Taline, Vaucheret Hervé
Abstract excerpt
Second-site mutagenesis was performed on the argonaute1-33 (ago1-33) hypomorphic mutant, which exhibits reduced sense transgene posttranscriptional gene silencing (S-PTGS). Mutations in FIERY1, a positive regulator of the cytoplasmic 5'-to-3' EXORIBONUCLEASE4 (XRN4), and in SUPERKILLER3 (SKI3), a member of the SKI complex that threads RNAs directly to the 3'-to-5' exoribonuclease of the cytoplasmic exosome,...
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