Article
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope.
Nature communications - 24 Apr 2020
Jiang Tingting, Henderson Jordana M, Coote Kevin, Cheng Yi, Valley Hillary C, Zhang Xiao-Ou, Wang Qin, Rhym Luke H, Cao Yueying, Newby Gregory A, Bihler Hermann, Mense Martin, Weng Zhiping, Anderson Daniel G, McCaffrey Anton P, Liu David R, Xue Wen
Abstract excerpt
CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires cellular exposure to levels of base editors that can be difficult to attain in hard-to-transfect cells or in vivo. Here we engineer a chemically modified mRNA-encoded adenine base editor that mediates robust editing at various cellular...
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