Article

<i>In situ</i> targeted mutagenesis of gut bacteria

2022-09-30

Abstract excerpt

Microbiome research is revealing a growing number of bacterial genes that impact our health. While CRISPR-derived tools have shown great success in editing disease-driving genes in human cells, we currently lack the tools to achieve comparable success for bacterial targets. Here we engineer a phage-derived particle to deliver a base editor and modify E. coli colonizing the mouse gut. This was achieved using a non...

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Identifiers and source

Literature Corpus work: 855413a1-30eb-5c44-95ca-8696856b9515
DOI: 10.1101/2022.09.30.509847

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