Article

Programmable conjugative CRISPR interference targeting genotoxin in the gut

2025-10-14

Abstract excerpt

<title>Abstract</title> <p>Among microbially derived metabolites that influence host disease, colibactin garners increasing attention for its roles in the rising incidence of early-onset colorectal cancer. Produced by pks⁺ Escherichia coli, colibactin is a potent genotoxin, yet no approved therapeutics directly suppress it. Here, we engineered a self-transmissible conjugative plasmid to deliver CRISPR interferenc...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 480cc501-2e29-5448-a865-059217dc3f1c
DOI: 10.21203/rs.3.rs-7761902/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Programmable conjugative CRISPR interference targeting genotoxin in the gutDOI 10.21203/rs.3.rs-7761902/v1Read

Programmable conjugative CRISPR interference targeting genotoxin in the gutSemantic proximity Read

Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity Read

Targeted-Antibacterial-Plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activitySemantic proximity Read

<i>Enterococcus faecalis</i> CRISPR-Cas is a robust barrier to conjugative antibiotic resistance dissemination in the murine intestineSemantic proximity Read

pGGTOX: a versatile plasmid platform for recombination-based genome editing across <i>Enterobacteriaceae</i>Semantic proximity Read

An attenuated CRISPR-Cas system in <i>Enterococcus faecalis</i> permits DNA acquisitionSemantic proximity Read

Factors affecting CRISPR-Cas defense against antibiotic resistance plasmids harbored by <i>Enterococcus faecalis</i> laboratory model strains and clinical isolatesSemantic proximity Read

<i>De novo</i> synthesis of a conjugative system from human gut metagenomic data for targeted delivery of Cas9 antimicrobialsSemantic proximity Read

Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical ImplicationsSemantic proximity Read

<i>Enterococcus faecalis</i> strains with compromised CRISPR-Cas defense emerge under antibiotic selection for a CRISPR-targeted plasmidSemantic proximity Read

Mechanistic dissection unmasks colibactin as a prevalent mutagenic driver of cancer.Semantic proximity Read

Multidrug-Resistant Enterococci Lack CRISPR- <i>cas</i>Semantic proximity Read