Article

Programmable conjugative CRISPR interference targeting genotoxin in the gut

2025-10-13

Abstract excerpt

Among microbially derived metabolites that influence host disease, colibactin garners increasing attention for its roles in the rising incidence of early-onset colorectal cancer. Produced by pks ⁺ Escherichia coli , colibactin is a potent genotoxin, yet no approved therapeutics directly suppress it. Here, we engineered a self-transmissible conjugative plasmid to deliver CRISPR interference (CRISPRi) into multipl...

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Literature Corpus work: baa7a166-d407-51c3-bf32-5dd5e24cb0df
DOI: 10.1101/2025.10.13.682122

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