Article

Phage-delivered CRISPR-Cas9 for strain-specific depletion and genomic deletions in the gut microbiome

2020-07-09

Abstract excerpt

Mechanistic insights into the role of the human microbiome in the predisposition to and treatment of disease are limited by the lack of methods to precisely add or remove microbial strains or genes from complex communities. Here, we demonstrate that engineered bacteriophage M13 can be used to deliver DNA to Escherichia coli within the mouse gastrointestinal (GI) tract. Delivery of a programmable exogenous CRISPR-...

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Literature Corpus work: 0c5f42d8-926f-5cee-991c-ddab77c4c0a7
DOI: 10.1101/2020.07.09.193847

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